Police say the confrontation happened in a coffee shop at Nobby Beach, near Miami, about 10.45am (AEST) on Tuesday.
A group of men were having coffee when three men got out of a small white car and approached them.
There was a confrontation and chairs were thrown as other cafe customers looked on.
No one was injured.
It's the third altercation between bikie gangs in public since Friday.
Another bikie brawl on Gold Coast as rivals clash at Smoothie Shack at Nobbys Beach, Gold Coast
- The Courier-Mail
- October 01, 2013
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Massive bikie crackdown on Gold Coast 0:30
Smoothie Shack owner Amanda Parker said a window was smashed in the melee.
She said the attack happened after some of her patrons were 'ambushed' as they sat eating breakfast, and as her nine-year-old daughter was stocking the fridge at the front of the cafe.
She said a one group of men sitting at another table appeared to call in a carload of four other men who arrived on the scene and started a fight.
"It seemed to be a bit of a drive-by ambush," she said.
"Four men got out of a car and it was on. It's not what you expect to happen in Nobby Beach - it doesn't seem to be one of those places where people have aggression."
Ms Parker said the drama lasted about five minutes but fortunately, no-one was hurt.
Police superintendent Ben Hanbidge said the melee was believed to be outlaw bikie gang-related.
"We've reviewed CCTV footage and it looks as though a few chairs and tables were thrown around the place," he said.
"It doesn't look as though anyone was seriously injured but certainly some members of the public were in there and they had to duck for cover.
"It's certainly disappointing to see so soon after what happened on Friday night in Broadbeach and we're making every effort to identify the people involved in this."
The bikies fled the scene, heading south on the Gold Coast Highway in two cars. One was a black Toyota Corolla and a white Holden sedan.
The shop is believed to be a known hangout for the Hells Angels.